Tuesday, 31 May 2011

Periodic Acid-Schiff (PAS) Stain

Periodic-Acid Schiff (PAS) Stain:
  • Pathology stain based on the periodic acid (H+IO4-) oxidation (cis-diols to aldehydes) & Schiff-base reactions.
  • Will stain anything with a cis-dihydroxy group a purple-magenta colour.
  • Typically stains insoluble sugars.
  • PAS staining is mainly used for highlighting structures containing a high proportion of carbohydrate macromolecules.
Tissues & Structures Stained by PAS:
  • Alpha-1 protease inhibitor (antitrypsin)
  • Amyloids (weakly)
  • Basement membranes
  • Cartilage
  • Epithelial mucin (except some acid mucins)
  • Fungal walls
  • Glycogen
  • Reticulin
  • Type I collagen (weakly)
Tags: Basement Membrane - Carbohydrate - Cartilage - Collagen - Fungal Wall - Glycogen - Mucin - PAS - Periodic Acid-Schiff Stain - Reticulin - Sugars
Posted by Medicalchemy
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